WebOct 25, 2013 · Our results also show that while in MCF-7 cells rapamycin induces clonogenic death with an IC 50 of ~50 nM, in the MDA-MB-231 cells concentrations up to 2 µM did not significantly affect cell survival (Table S2 in File S1). eIF2α is a downstream target of rapamycin and irradiation. WebSep 16, 2024 · Head and neck squamous cell carcinoma (HNSCC) is a challenging cancer with little change in five-year overall survival rate of 50–60% over the last two decades. Radiation with or without platinum-based drugs remains the standard of care despite limited benefit and high toxicity. HNSCCs often overexpress epidermal growth factor receptor …
Clonogenic High-Throughput Assay for Screening Anti-Cancer
WebMechanistically, Dex increased superoxide and hydrogen peroxide production and augmented radiation-induced oxidative stress and cell death of myeloma cells. In contrast, Dex inhibited radiation-induced increase in pro-oxidant levels and enhanced the clonogenic survival in normal hematopoietic stem and progenitor cells. WebApr 8, 2024 · Lung cancer is the leading cause of cancer-related death, accounting for approximately 350 deaths per day . Non-small cell lung cancer (NSCLC) ... In the clonogenic assay, cells were suspended and ... samsung clothes dryers problems
Apoptosis and Clonogenic Cell Death in PC3 Human Prostate …
WebDec 1, 2014 · The accretion of AVO in surviving progenies of irradiated cells, and the increased incidence of clonogenic death after inhibition of vacuolar H+-ATPase suggest that formation of acidic organelles represents a novel defense mechanism against radiation damage. Expand. 1,009. PDF. View 2 excerpts, references background and methods ... WebMar 1, 2007 · Conversely, inheritance of wild-type p53 by prostate cancer cells lacking a functional allele of this gene (i.e., DU145) significantly increases clonogenic death through p53-dependent cellular senescence and apoptotic pathways. Our data provide evidence that mutations of even one p53 allele may be sufficient to alter their clonogenic fate. WebA clonogenic assay, also known as a colony formation assay. is an in vitro cell survival assay. It assesses the ability of single cells to survive and reproduce to form colonies1. This assay was first described in the 1950s, … samsung clothes washer app