WebS7 Fig: Examination of viral suppressors of RNA-silencing (VSR) in RTIV.(A) Schematic representation of the Agrobacterium infiltration.(B) 16C transgenic N. benthamiana were respectively co-infiltrated with GFP and P0, CP, MP or full length of RTIV. Empty vector and VSRs were used as negative and positive control, respectively. GFP was visualized … WebApr 16, 2010 · (A) Leaves of 16c GFP-transgenic plants were coinfiltrated with 35S-GFP and either empty vector, 35S-p19, 35S-myc-NtRDR1, 35S-NtΔRDR1, or 35S-NtRDR1m. Photographs were taken under UV light at 4 dpa. (B) RNA gel blot analysis of GFP mRNA and GFP -derived siRNA accumulation. 32 P-labeled GFP DNA or RNA probes were used.
The widely used Nicotiana benthamiana 16c line has …
WebMar 15, 2024 · The GFP16C-like systemic silencing phenotype was only observed in the two-copy line. The partial transposase had no impact on transgene expression level, … WebMar 28, 2024 · Furthermore, agroinfiltration assays in N. benthamiana wilt type or GFP-transgenic 16c indicated that ToCV CPm protein effectively suppressed local RNA silencing induced by single-stranded but not double-stranded RNA, which probably resulted from the activity of binding double-stranded but not single-stranded RNA by ToCV CPm protein. craft trail ledge
Tomato chlorosis virus CPm protein is a pathogenicity ... - PubMed
WebSep 24, 2011 · The transgenic GFP-expressing N. benthamiana line 16c used in this study has been previously described . Seeds (kindly provided by Professor D. Baulcombe, Sainsbury Laboratory, Norwich, UK) were sown in small pots with an artificial potting mix (50% sand and 50% peat moss) in a plant growth chamber at 20/24°C (night/day), 50% … WebOct 2, 2006 · To create a similar model for studies of glial cells in zebrafish, we have generated transgenic lines of zebrafish, Tg ( gfap: GFP), that express GFP driven by … WebIn this study, transgenic N. benthamiana (line 16c), which shows strong constitutive expression of the green fluorescent protein (GFP) (Brigneti et al. , 1998) and allows non-disruptive... dixon\\u0027s seafood shoppe peoria